“Argonne had this extremely powerful X-ray microscope, and it hadn’t been used for brain mapping yet, so we decided to try it out.” - Assistant Professor Bobby Kasthuri It leveraged existing advanced X-ray microscopy techniques at the Advanced Photon Source ( APS), a DOE Office of Science User Facility at Argonne, to bridge the gap between MRI and electron microscopy imaging, providing a viable pipeline for multiscale whole brain imaging within the same brain. The advance, which was published on June 9 in NeuroImage, will allow scientists to connect biomarkers at the microscopic and macroscopic level.
Department of Energy’s ( DOE) Argonne National Laboratory have imaged an entire mouse brain across five orders of magnitude of resolution, a step which researchers say will better connect existing imaging approaches and uncover new details about the structure of the brain. 1.Researchers at the University of Chicago and the U.S. Cell density is approximately equal to that of Fig. 1.įigure 5: Uninfected mouse L cells viewed with epifluorescence at 200x magnification.
1.įigure 4: Mouse L cells infected with a 1/100 viral dilution and viewed with epifluorescence at 200x magnification. The 10-fold viral dilution reduces the amount of infectious virus present, thereby reducing the number of infected mouse L cells and providing a field of view with a countable number of fluorescing cells. 1.įigure 3: Mouse L cells infected with a 1/10 viral dilution and viewed with epifluorescence at 200x magnification. This image shows the same field of view as Fig. A majority of the cells are infected and display a distinct cytoplasmic fluorescence. Cells infected with virus are first tagged with a rabbit anti-reovirus antibody and subsequently tagged with a secondary anti-rabbit antibody, which is fluorescently labeled. Maximal viral production within an infected cell occurs about 24 hours postentry.įigure 2: Infected mouse L cells viewed with epifluorescence at 200x magnification. After assembly of new viral particles, host cells will be lysed to release new infectious viral particles. Proteolysis of the outer-capsid proteins occurs via a two-step disassembly process and replication then occurs in the cytoplasm. Following virion attachment to cell surface receptors, reovirus particles enter the host cell through receptor-mediated endocytosis. Download the PowerPoint PowerPoint Contentsįigure 1: Infected mouse L cells viewed with bright-field microscopy at 200x magnification.
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